|
KMID : 1041220040460020072
|
|
Journal of Sericultural and Entomological Science
2004 Volume.46 No. 2 p.72 ~ p.76
|
|
|
Effect of Bombyx mori, Antheraea Yamamai and Antheraea pernyi Silk Protein in Skin Fibroblast Cell Proliferation After Injury
|
|
Han Sang-Mi
Lee Kwang-Gill
Yeo Joo-Hong
Kwon Hae-Yong
Woo Soon-Ok
Paik Ha-Ju
Park Kwan-Kyu
|
|
|
Abstract
|
|
|
|
We have studied the effect of silk proteins to the cell proliferation of human skin fibroblast cells (CCD-986sk) after injury. Silk proteins were extracted treatment with enzyme or NaOH solution from raw silk and culled-cocoon shell of Bombyx mori, Antheraea yamamai and A. pernyi. The cell proliferation after in vitro injury are increased in treatment by Bombyx mori (BM-1,2), Antheraea yamami (AY-1,2) and A. pernyi (AP-1,2). The silk protein fractions-treated cells exhibited proliferation in a dose dependent between $0.1\;{\mu}g/ml$ and $10\;{\mu}g/ml$. But, the macrophage, RAW 264. 7 cell viability was unaffected by the silk protein fractions by MTT assay. The molecular weights of the silk protein fractions were from 300-600 to 900-1500. These results results that the silk protein fractions may function through skin fibroblast proliferation.
|
|
|
KEYWORD
|
|
|
Bombyx, mori, Antheraea Yamamai, Antheraea pernyi, skin fibroblast cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|